- Industrial Enzyme Engineering
- G-protein coupled receptors (GPCRs) in infection and disease.
- Mechanism of fungal biocontrol
On the one hand we are engaged in industrial enzyme engineering toward the development of novel bioprocessing technologies to apply to industrial waste streams for the production of value added products in the mining, forestry, textiles and feed/food industries.
On the other hand our lab is investigating mechanistic aspects of fungal pathogenesis and related fungal biocontrol. This includes a particular focus on Fusarium graminearum, a devastating fungal pathogen of cereal crops that both decreases grain quality and yield as well as deposits mycotoxins. Fusarium has become significantly more prevalent in Canada recently, likely related to climate change. Toward addressing Fusarium, we are also studying the mechanisms underlying the fungal biocontrol agent Clonostachy rosea, shown to have growth inhibition activity toward Fusarium. We use genetic, transcriptomic, metabolomic, biochemical and biophysical methods to probe fungal perception and signal transduction as well as metabolic pathways underlying the pathogenic and biocontrol functionalities of these fungal species. Assays use a variety of different analytical methods such as liquid chromatography, mass spectroscopy, photospectroscopy and nuclear magnetic resonance for metabolite analyses. We also use protein crystallography to better understand protein-structure function relationships.
Publications, Protein Structures and Patents
Our 3 latest publications include:
Mohapatra DP, Robinson KA, Huang F, Kirpalani D, Loewen MC (2022) Insights into Increasing Selenate Reductase Enzyme Activity in the Presence of Nitrogen-Doped Graphite Electrodes for Selenium Effluent Treatment. Water 14:931. [online]
Buhrow LM, Liu Z, Cram D, Sharma T, Foroud NA, Pan Y, Loewen MC. (2021)Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge.BMC Genomics 22:798. [online]
Demissie ZA, Robinson KA, Loewen MC (2021) Draft genome resources for the plant-beneficial fungi Clonostachys rosea strains ACM941 and 88-710. Molecular Plant-Microbe Interactions, doi: 10.1094/MPMI-10-20-0294-A. [online]
Please see our full list of publications in PubMed
Please also enjoy our 6 most recent protein structures listed in the RCSB: 7U1H, 7U1I, 7U1J, 5V2D, 4MKV, 4HHH.
Please also see our patent at USPTO
Current Group Members
Michele C. Loewen
Senior Research Officer
Adjunct Professor – QueensU
Adjunct Professor – uOttawa
Pooja Sridhar (Ph.D. Student; QueensU)
Kelly Robinson (Technical Officer; NRC)
Tanya Sharma (Ph.D. Student; uOttawa)
Joshua Lister (M.Sc. Student; uOttawa)
Peter Adewale (Research Associate; NRC)
Antony St-Jacques (Research Associate; NRC)
Deandre Chevannes (M.Sc. Student; uOttawa)
Joanna Stanczyk (Coop Student; QueensU)
Yutong Zhang (M.Sc. Student; uOttawa)
Idin Lantz (Undergraduate Project Student; uOttawa)
Luana Porto (Post-Doctoral Associate; uOttawa)
Please see also our lab Alumni